Ascorbic Acid Synthesis from D-Glucose-%C1* in the Liver of the Intact Rat
نویسنده
چکیده
Evidence that glucose1 is the ultimate g-carbon precursor of ascorbic acid in the rat was first obtained by Jackel, Mosbach, Burns, and King (1). They administered uniformly labeled glucose-C** to Chloretone-treated rats and recovered uniformly labeled ascorbic acid from the urine. Subsequently, Horowitz, Doerschuk, and King (2) and Burns and Mosbach (3) discovered that when glucose-l-C14 was administered to Chloretone-treated or normal rats, the urinary ascorbic acid was labeled primarily in C-6. Experiments employing glucose-6-Cl4 in Chloretonetreated rats resulted in urinary ascorbic acid labeled primarily in C-l (4). These tracer studies and the nonisotopic experiments of Isherwood, Chen, and Mapson (5) led to postulation of the following scheme of ascorbic acid biosynthesis in the rat:
منابع مشابه
Ascorbic acid synthesis from D-glucose-2-C14 in the liver of the intact rat.
Evidence that glucose1 is the ultimate g-carbon precursor of ascorbic acid in the rat was first obtained by Jackel, Mosbach, Burns, and King (1). They administered uniformly labeled glucose-C** to Chloretone-treated rats and recovered uniformly labeled ascorbic acid from the urine. Subsequently, Horowitz, Doerschuk, and King (2) and Burns and Mosbach (3) discovered that when glucose-l-C14 was a...
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Previous studies showed that the intact carbon chain of glucose can serve as a precursor for the synthesis of ascorbic acid in the rat (l-3). Evidence for such a precursor relationship came from experiments in which glucose tracers labeled in carbon 1 and carbon 6 were administered to rats, and the L-ascorbic acid isolated from urine was labeled predominantly in carbon 6 and carbon 1, respectiv...
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In exploring the mechanism of biosynthesis of ascorbic acid by the rat, King and his coworkers (Musulin, Tully, Longenecker & King, 1939; Longenecker, Musulin, Tully & King, 1939; Longenecker, Fricke & King, 1940) observed that rats treated with Chloretone (1:1:1-trichloro-2methylpropan-2-ol) excreted more ascorbic acid than did the controls. By employing Chloretonized rats and isotopically lab...
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